THIS COURSE IS NOW FULLY BOOKED
The STEM partnerships team at St Paul’s holds a range of workshops, lectures and extracurricular courses throughout the academic year primarily aimed at students in Years 10 and 12.
All students from our maintained sector partner schools are welcome to apply for these courses and attend for free, although a small deposit may be requested which is refundable upon the student completing the course.
This is a three week long course hosted on consecutive Saturdays (15 Jan, 22 Jan and 29 Jan). The aim of this course is to provide keen and talented scientists with the opportunity to gain hands-on experience of working with molecular biology equipment normally found in a research lab. This is a great chance for students to learn about sophisticated molecular biology techniques, and will be invaluable experience for those considering biology, biochemistry and related degrees.
With the teaching expertise and laboratory facilities available at St Paul’s, participants will cover:
SESSION 1: SATURDAY 22 JANUARY 2021
Use of Gilson micropipettes – ‘the tools of the trade’
Genetic Transformation of bacteria with the Green Fluorescent Protein (GFP) gene
Expression of GPP in bacteria
Agarose gel electrophoresis to examine plasmid DNA
SESSION 2: SATURDAY 29 JANUARY 2021
Analysis of the results of the expression of GFP in bacteria
SDS polyacrylamide gel electrophoresis to examine protein content of bacterial cells
Set up of bacterial cultures for over expression of GFP protein for the next week.
SESSION 1: SATURDAY 5 FEBRUARY 2021
Purification of GFP protein from bacterial cells extracts
Please arrive at 9.15am to register for a prompt start.
All sessions run from 9.30am – 12.20pm with a short break halfway through the morning.